abstract：:Many extracellular signals are transmitted to the interior of the cell by receptors with seven membrane-spanning helices that trigger their effects by means of heterotrimeric guanine-nucleotide-binding regulatory proteins (G proteins). These G-protein-coupled receptors (GPCRs) control various physiological functions i...

journal_title：Nature cell biology

authors： Cvejic S,Zhu Z,Felice SJ,Berman Y,Huang XY

更新日期：2004-06-01 00:00:00

abstract：:Phosphoinositides (PIs) undergo phosphorylation/dephosphorylation cycles through organelle-specific PI kinases and PI phosphatases that lead to distinct subcellular distributions of the individual PI species. Specific PIs control the correct timing and location of many trafficking events. Their ultimate mode of action...

journal_title：Nature cell biology

authors： De Matteis MA,Godi A

更新日期：2004-06-01 00:00:00

abstract：:The molecular mechanisms underlying the formation of carriers trafficking from the Golgi complex to the cell surface are still ill-defined; nevertheless, the involvement of a lipid-based machinery is well established. This includes phosphatidylinositol 4-phosphate (PtdIns(4)P), the precursor for phosphatidylinositol 4...

journal_title：Nature cell biology

authors： Godi A,Di Campli A,Konstantakopoulos A,Di Tullio G,Alessi DR,Kular GS,Daniele T,Marra P,Lucocq JM,De Matteis MA

更新日期：2004-05-01 00:00:00

abstract：:During the development of multicellular organisms, concerted actions of molecular signalling networks determine whether cells undergo proliferation, differentiation, death or ageing. Here we show that genetic inactivation of the stress signalling kinase, MKK7, a direct activator of JNKs in mice, results in embryonic l...

journal_title：Nature cell biology

authors： Wada T,Joza N,Cheng HY,Sasaki T,Kozieradzki I,Bachmaier K,Katada T,Schreiber M,Wagner EF,Nishina H,Penninger JM

更新日期：2004-03-01 00:00:00

abstract：:For accurate segregation of chromosomes during cell division, microtubule fibres must attach sister kinetochores to opposite poles of the mitotic spindle (bi-orientation). Aurora kinases are linked to oncogenesis and have been implicated in the regulation of chromosome-microtubule attachments. Although loss of Aurora ...

journal_title：Nature cell biology

authors： Lampson MA,Renduchitala K,Khodjakov A,Kapoor TM

更新日期：2004-03-01 00:00:00

abstract：:NF-kappa B/Rel transcription factors control apoptosis, also known as programmed cell death. This control is crucial for oncogenesis, cancer chemo-resistance and for antagonizing tumour necrosis factor alpha (TNFalpha)-induced killing. With regard to TNFalpha, the anti-apoptotic activity of NF-kappa B involves suppres...

journal_title：Nature cell biology

authors： Papa S,Zazzeroni F,Bubici C,Jayawardena S,Alvarez K,Matsuda S,Nguyen DU,Pham CG,Nelsbach AH,Melis T,De Smaele E,Tang WJ,D'Adamio L,Franzoso G

更新日期：2004-02-01 00:00:00

abstract：:The accurate spatial and temporal coordination of cell polarization with DNA replication and segregation guarantees the fidelity of genetic transmission. Here we report that in Saccharomyces cerevisiae, a build-up or burst of G1 cyclin-dependent kinase (CDK) activity through activation of the cyclin genes CLN1,2 and P...

journal_title：Nature cell biology

authors： Moffat J,Andrews B

更新日期：2004-01-01 00:00:00

abstract：:Heterochromatin performs a central role in chromosome segregation and stability by promoting cohesion at centromeres. Establishment of both heterochromatin-mediated silencing and cohesion requires passage through S phase, although the mechanism is unknown. Here we demonstrate that Schizosaccharomyces pombe Hsk1 (CDC7)...

journal_title：Nature cell biology

authors： Bailis JM,Bernard P,Antonelli R,Allshire RC,Forsburg SL

更新日期：2003-12-01 00:00:00

abstract：:Genomic integrity is maintained by checkpoints that guard against undesired replication after DNA damage. Here, we show that CDT1, a licensing factor of the pre-replication complex (preRC), is rapidly proteolysed after UV- or gamma-irradiation. The preRC assembles on replication origins at the end of mitosis and durin...

journal_title：Nature cell biology

authors： Higa LA,Mihaylov IS,Banks DP,Zheng J,Zhang H

更新日期：2003-11-01 00:00:00

abstract：:The formation of branched epithelial networks is fundamental to the development of many organs, such as the lung, the kidney or the vasculature. Little is known about the mechanisms that control cell rearrangements during tubulogenesis and regulate the size of individual tubes. Recent studies indicate that whereas the...

journal_title：Nature cell biology

authors： Jaźwińska A,Ribeiro C,Affolter M

更新日期：2003-10-01 00:00:00

abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-activated chloride channel expressed in a wide variety of epithelial cells, mutations of which are responsible for the hallmark defective chloride secretion observed in cystic fibrosis (CF). Although CFTR has been implicated in bicarbonate secretion,...

journal_title：Nature cell biology

authors： Wang XF,Zhou CX,Shi QX,Yuan YY,Yu MK,Ajonuma LC,Ho LS,Lo PS,Tsang LL,Liu Y,Lam SY,Chan LN,Zhao WC,Chung YW,Chan HC

更新日期：2003-10-01 00:00:00

abstract：:Cholesterol is a structural component of animal membranes that influences fluidity, permeability and formation of lipid microdomains. It is also a precursor to signalling molecules, including mammalian steroid hormones and insect ecdysones. The nematode Caenorhabditis elegans requires too little cholesterol for it to ...

journal_title：Nature cell biology

authors： Kurzchalia TV,Ward S

更新日期：2003-08-01 00:00:00

abstract：:The actin filament (F-actin) cytoskeleton associates dynamically with the plasma membrane and is thus ideally positioned to participate in endocytosis. Indeed, a wealth of genetic and biochemical evidence has confirmed that actin interacts with components of the endocytic machinery, although its precise function in en...

journal_title：Nature cell biology

authors： Sokac AM,Co C,Taunton J,Bement W

更新日期：2003-08-01 00:00:00

abstract：:Many pro-apoptotic signals activate caspase-9, an initiator protease that activates caspase-3 and downstream caspases to initiate cellular destruction. However, survival signals can impinge on this pathway and suppress apoptosis. Activation of the Ras-Raf-MEK-ERK mitogen-activated protein kinase (MAPK) pathway is asso...

journal_title：Nature cell biology

authors： Allan LA,Morrice N,Brady S,Magee G,Pathak S,Clarke PR

更新日期：2003-07-01 00:00:00

abstract：:NMDA (N-methyl-D-aspartate) receptors (NMDARs) are targeted to dendrites and anchored at the post-synaptic density (PSD) through interactions with PDZ proteins. However, little is known about how these receptors are sorted from the endoplasmic reticulum and Golgi apparatus to the synapse. Here, we find that synapse-as...

journal_title：Nature cell biology

authors： Sans N,Prybylowski K,Petralia RS,Chang K,Wang YX,Racca C,Vicini S,Wenthold RJ

更新日期：2003-06-01 00:00:00

abstract：:Endocytosis of cell surface receptors is an important regulatory event in signal transduction. The transforming growth factor beta (TGF-beta) superfamily signals to the Smad pathway through heteromeric Ser-Thr kinase receptors that are rapidly internalized and then downregulated in a ubiquitin-dependent manner. Here w...

journal_title：Nature cell biology

authors： Di Guglielmo GM,Le Roy C,Goodfellow AF,Wrana JL

更新日期：2003-05-01 00:00:00

abstract：:In chromaffin cells, exocytosis of single granules and properties of the fusion pore--the first connection between vesicular lumen and extracellular space --can be studied by cell-attached patch amperometry, which couples patch-clamp capacitance measurements with simultaneous amperometric recordings of transmitter rel...

journal_title：Nature cell biology

authors： Dernick G,Alvarez de Toledo G,Lindau M

更新日期：2003-04-01 00:00:00

abstract：:Clathrin-coated vesicles (CCVs) are a central component of endocytosis and traffic between the trans-Golgi network (TGN) and endosomes. Although endocytic CCV formation is well characterized, much less is known about CCV formation at internal membranes. Here we describe two epsin amino-terminal homology (ENTH) domain-...

journal_title：Nature cell biology

authors： Duncan MC,Costaguta G,Payne GS

更新日期：2003-01-01 00:00:00

abstract：:Many classical biologists working in Antarctica have 'gone molecular' to study the physiological basis for life on or below the ice. Investigating the remarkable adaptations of specific cells, organelles and molecules to this extreme environment can provide new perspectives on the processes studied in conventional exp...

journal_title：Nature cell biology

authors： Römisch K,Matheson T

更新日期：2003-01-01 00:00:00

abstract：:Activation of the ataxia telangiectasia mutated (ATM) kinase triggers diverse cellular responses to ionizing radiation (IR), including the initiation of cell cycle checkpoints. Histone H2AX, p53 binding-protein 1 (53BP1) and Chk2 are targets of ATM-mediated phosphorylation, but little is known about their roles in sig...

journal_title：Nature cell biology

authors： Fernandez-Capetillo O,Chen HT,Celeste A,Ward I,Romanienko PJ,Morales JC,Naka K,Xia Z,Camerini-Otero RD,Motoyama N,Carpenter PB,Bonner WM,Chen J,Nussenzweig A

更新日期：2002-12-01 00:00:00

abstract：:Assisted reproductive technologies (ART) are exceptional among clinical therapies, as unlike most medical procedures, ART have generational consequences. Further, human embryo research in the US has been sponsored solely by the private sector and, until recent biotechnology forays into human embryonic stem cell (hESC)...

journal_title：Nature cell biology

authors： Schatten GP

更新日期：2002-10-01 00:00:00

abstract：:The formation of axon trajectories requires integration of local adhesive interactions with directional information from attractive and repulsive cues. Here, we show that these two types of information are functionally integrated; activation of the transmembrane receptor Roundabout (Robo) by its ligand, the secreted r...

journal_title：Nature cell biology

authors： Rhee J,Mahfooz NS,Arregui C,Lilien J,Balsamo J,VanBerkum MF

更新日期：2002-10-01 00:00:00

abstract：:The migration of border cells during Drosophila melanogaster oogenesis is a simple and powerful system for studying invasive cell migration in vivo. Border cells are somatic cells that delaminate from the follicular epithelium of an egg chamber and invade the germ line cluster. They migrate between the nurse cells to ...

journal_title：Nature cell biology

authors： Fulga TA,Rørth P

更新日期：2002-09-01 00:00:00

abstract：:Connective-tissue growth factor (CTGF) is a secreted protein implicated in multiple cellular events including angiogenesis, skeletogenesis and wound healing. It is a member of the CCN family of secreted proteins, named after CTGF, cysteine-rich 61 (CYR61), and nephroblastoma overexpressed (NOV) proteins. The molecular...

journal_title：Nature cell biology

authors： Abreu JG,Ketpura NI,Reversade B,De Robertis EM

更新日期：2002-08-01 00:00:00

abstract：:Inhibitors of apoptosis (IAPs) inhibit caspases, thereby preventing proteolysis of apoptotic substrates. IAPs occlude the active sites of caspases to which they are bound and can function as ubiquitin ligases. IAPs are also reported to ubiquitinate themselves and caspases. Several proteins induce apoptosis, at least i...

journal_title：Nature cell biology

authors： Holley CL,Olson MR,Colón-Ramos DA,Kornbluth S

更新日期：2002-06-01 00:00:00

abstract：:The store-operated calcium channel (SOC) located in the plasma membrane (PM) mediates capacitative entry of extracellular calcium after depletion of intracellular calcium stores in the endoplasmic or sarcoplasmic reticulum (ER/SR). An intimate interaction between the PM and the ER/SR is essential for the operation of ...

journal_title：Nature cell biology

authors： Pan Z,Yang D,Nagaraj RY,Nosek TA,Nishi M,Takeshima H,Cheng H,Ma J

更新日期：2002-05-01 00:00:00

abstract：:Chromophore-assisted laser inactivation (CALI) is a light-mediated technique used to selectively inactivate proteins within cells. Here, we demonstrate that GFP can be used as a CALI reagent to locally inactivate proteins in living cells. We show that focused laser irradiation of EGFP-alpha-actinin expressed in Swiss ...

journal_title：Nature cell biology

authors： Rajfur Z,Roy P,Otey C,Romer L,Jacobson K

更新日期：2002-04-01 00:00:00

abstract：:Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...

journal_title：Nature cell biology

authors： Evangelista M,Pruyne D,Amberg DC,Boone C,Bretscher A

更新日期：2002-03-01 00:00:00

abstract：:In Huntington disease, polyglutamine expansion of the protein huntingtin (Htt) leads to selective neurodegenerative loss of medium spiny neurons throughout the striatum by an unknown apoptotic mechanism. Binding of Hip-1, a protein normally associated with Htt, is reduced by polyglutamine expansion. Free Hip-1 binds t...

journal_title：Nature cell biology

authors： Gervais FG,Singaraja R,Xanthoudakis S,Gutekunst CA,Leavitt BR,Metzler M,Hackam AS,Tam J,Vaillancourt JP,Houtzager V,Rasper DM,Roy S,Hayden MR,Nicholson DW

更新日期：2002-02-01 00:00:00

abstract：:Endoplasmic reticulum (ER)-associated protein degradation by the ubiquitin-proteasome system requires the dislocation of substrates from the ER into the cytosol. It has been speculated that a functional ubiquitin proteasome pathway is not only essential for proteolysis, but also for the preceding export step. Here, we...

journal_title：Nature cell biology

authors： Jarosch E,Taxis C,Volkwein C,Bordallo J,Finley D,Wolf DH,Sommer T

更新日期：2002-02-01 00:00:00

abstract：:It has long-been accepted that normal somatic cells have intrinsic mechanisms that limit their proliferative lifespan. Recent work has now challenged this view by demonstrating that extrinsic factors might be determining proliferative potential. ...

journal_title：Nature cell biology

authors： Lloyd AC

更新日期：2002-02-01 00:00:00

abstract：:Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...

journal_title：Nature cell biology

authors： Evangelista M,Pruyne D,Amberg DC,Boone C,Bretscher A

更新日期：2002-01-01 00:00:00

abstract：:Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously im...

journal_title：Nature cell biology

authors： Hetzer M,Meyer HH,Walther TC,Bilbao-Cortes D,Warren G,Mattaj IW

更新日期：2001-12-01 00:00:00

abstract：:Vaccinia virus, a close relative of the causative agent of smallpox, exploits actin polymerization to enhance its cell-to-cell spread. We show that actin-based motility of vaccinia is initiated only at the plasma membrane and remains associated with it. There must therefore be another form of cytoplasmic viral transpo...

journal_title：Nature cell biology

authors： Rietdorf J,Ploubidou A,Reckmann I,Holmström A,Frischknecht F,Zettl M,Zimmermann T,Way M

更新日期：2001-11-01 00:00:00

abstract：:Chaperone proteins are thought to promote the correct folding and assembly of newly synthesized proteins and to facilitate restoration of the folded state under environmental conditions that favour protein denaturation. They are among the most ubiquitous and highly conserved of all proteins. The eukaryotic endoplasmic...

journal_title：Nature cell biology

authors： Randow F,Seed B

更新日期：2001-10-01 00:00:00

abstract：:The precise regulation of growth factor signalling is crucial to the molecular control of development in Drosophila. Post-translational modification of signalling molecules is one of the mechanisms that modulate developmental signalling specificity. We describe a new gene, fringe connection (frc), that encodes a nucle...

journal_title：Nature cell biology

authors： Selva EM,Hong K,Baeg GH,Beverley SM,Turco SJ,Perrimon N,Häcker U

更新日期：2001-09-01 00:00:00

abstract：:Current models for the export of messenger RNA share the notion that the highly abundant class of nuclear RNA-binding proteins--the hnRNP proteins--have a key role in exporting RNA. But recent studies have led to a new understanding of several non-hnRNP proteins, including SR proteins and the conserved mRNA export fac...

journal_title：Nature cell biology

authors： Reed R,Magni K

更新日期：2001-09-01 00:00:00

abstract：:The importance of herpes simplex viruses (HSV) as human pathogens and the emerging prospect of using mutant derivatives of HSV-1 as potential anti-cancer therapeutics have necessitated a thorough investigation into the molecular basis of host-cell permissiveness to HSV. Here we show that NIH-3T3 cells transformed with...

journal_title：Nature cell biology

authors： Farassati F,Yang AD,Lee PW

更新日期：2001-08-01 00:00:00

abstract：:Parallel quantification of large numbers of messenger RNA transcripts using microarray technology promises to provide detailed insight into cellular processes involved in the regulation of gene expression. This should allow new understanding of signalling networks that operate in the cell and of the molecular basis an...

journal_title：Nature cell biology

authors： Schulze A,Downward J

更新日期：2001-08-01 00:00:00

abstract：:PX domains are found in a variety of proteins that associate with cell membranes, but their molecular function has remained obscure. We show here that the PX domains in p47phox and p40phox subunits of the phagocyte NADPH oxidase bind to phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4)P(2)) and phosphatidylinositol-3...

journal_title：Nature cell biology

authors： Kanai F,Liu H,Field SJ,Akbary H,Matsuo T,Brown GE,Cantley LC,Yaffe MB

更新日期：2001-07-01 00:00:00